Cloning of glucanase gene from Bacillus pumilus C 9 and its expression in Saccharomyces cerevisiae

Key words:  glucanase  rDNA  Saccharomyces cerevisiae  multicopy integration

Abstract:

The glucanase gene was obtained from Bacillus pumilus C 9, the open reading frame of glucanase consists of 1980 nucleotides encoding a protein of 559 amino acids with a predicted signal peptide of 29 amino acids at the N terminal. To achieve the stable and high efficient expression in Saccharomyces cerevisiae, a multicopy integration plasmid was constructed by introducing the rDNA sequence into the plasmid YIp5. The recombinant Saccharomyces cerevisiae could grow on the plates with carboxymethyl cellulose as the sole carbon source.