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Study on the activation Geobacillus species in Zhan 3 blocks of Shengli Oilfield
LI Caifeng1,2, LI Yang3, WU Xinyu4, CAO Yanbin2,WANG Weidong2, BAO Mutai1
(1.College of Chemistry & Chemical Engineering in Ocean University of China, Qingdao 266100, China;2.Shengli Oil Production Research Institution, SINOPEC, Dongying 257000, China;3.China Petroleum & Chemical Corporation, Beijing 100728, China;4.College of information and Control Engineering in China University of Petroleum, Qingdao 266580, China)
Abstract:
The microbial community structure in Zhan 3 block reservoir of Shengli Oilfield was analyzed by means of 16S rRNA clone library. The indigenous microorganism in Zhan 3 block reservoir was activated by adding different activators. And the emulsifying ability, the ability of producing surfactant, and the analysis of microbial community structure for the activated samples were studied. The physical simulation flooding experiments were also carried out. The results show that Geobacillus accounts for 2% of the total sequences in Zhan 3 reservoir samples, which is the key species for producing surfactant and playing the function of emulsification. It could be selectively activated and becomes the dominant species by adding proper activators. The indigenous microorganism could enhance oil recovery up to 10.8% based on water flooding at the Geobacillus activator formula in the reservoir conditions.
Key words:  Geobacillus  emulsifying ability  activation  indigenous microorganism  microbial population