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Study on catalytic oxidation performance of hemin dispersed by n-dodecyl-β-D-maltopyranoside micelles |
WANG Xiaoqiang, WANG Chao, PAN Meihong, CHEN Han
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(Protein Molecular Engineering Laboratory of Bioengineering and Biotechnology Center in China University of Petroleum, Qingdao 266580, China)
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Abstract: |
The catalytic oxidation property of bionic composites formed by hemin and nonionic surfactant n-Dodecyl-β-D-maltopyranoside (DDM)was studied. The dynamic light scattering (DLS) and UV-vis spectrophotometry were used to characterize the micellization behavior and the DDM dispersion effect on hemin. And the catalytic oxidation property of the composite system for the model compound Orange Ⅱ was studied, in which the H2O2 was used as an oxidant substrate. The results show that the catalytic reaction system obeys the Michaelis-Menten equation of enzymatic catalysis. The Michaelis-Mentenconstant(Km) and maximum velocity(vmax) of Orange Ⅱ are estimated by Lineweaver-Burk model to be 0.1 mmol/L and 6.0×10-4 mmol/(L·min), respectively. The Km and vmax of the H2O2 are 4.6 mmol/L and 9.2×10-4 mmol/(L·min). In comparison with natural peroxidases, the hemin-DDM micelles catalytic system has characteristics of easy preparation, low cost and good stability. |
Key words: hemin n-dodecyl-β-D-maltopyranoside Orange Ⅱ catalytic oxidation |
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