Study on catalytic oxidation performance of hemin dispersed by n-dodecyl-β-D-maltopyranoside micelles

Key words:  hemin  n-dodecyl-β-D-maltopyranoside  Orange Ⅱ  catalytic oxidation

Abstract:

The catalytic oxidation property of bionic composites formed by hemin and nonionic surfactant n-Dodecyl-β-D-maltopyranoside (DDM)was studied. The dynamic light scattering (DLS) and UV-vis spectrophotometry were used to characterize the micellization behavior and the DDM dispersion effect on hemin. And the catalytic oxidation property of the composite system for the model compound Orange Ⅱ was studied, in which the H₂O₂ was used as an oxidant substrate. The results show that the catalytic reaction system obeys the Michaelis-Menten equation of enzymatic catalysis. The Michaelis-Mentenconstant(K_m) and maximum velocity(v_max) of Orange Ⅱ are estimated by Lineweaver-Burk model to be 0.1 mmol/L and 6.0×10^-4 mmol/(L·min), respectively. The K_m and v_max of the H₂O₂ are 4.6 mmol/L and 9.2×10^-4 mmol/(L·min). In comparison with natural peroxidases, the hemin-DDM micelles catalytic system has characteristics of easy preparation, low cost and good stability.