Bioremediation of 16 EPA-PAHs combined contaminated-soil with microbial consortium

Key words:  polycyclic aromatic hydrocarbons  contaminated-soil  microbial consortium  biodegradation

Abstract:

For bioremediation of polycyclic aromatic hydrocarbons (PAHs) combined contaminated-soil, a microbial consortium and three strains were isolated from PAHs contaminated-soil containing sixteen US Environmental Protection Agency pri_ ority control PAHs. Denaturing gradient gel electrophoresis (DGGE) was used to analyze the structure of the microbial consortium ,and the biodegradation effect of PAHs mixtures with the inoculation of mixed microbial consortium and single strain was studied. The results show that the consortium is mainly composed of three isolated strains and five uncultured bacterias. After 30 days bioremediation, the degradation efficiency of total PAHs by microbial consortium (54. 17% ) is higher than any single strain's (28. 40% ,31. 95% ,24. 64% ) Microbial consortium shows a great advantage in degradation of high relative molecular mass PAHs, and the degradation efficiencies of four-rings, five-rings and six-rings PAHs are 71. 26%，39. 76%， 42. 86%，respectively. Microbial consortium is capable of degrading PAHs which can avoid the loss of key strains and has a good application prospect in the bioremediation of PAHs contaminated-soil.