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Interaction between in-situ oil reservoir microorganisms and minerals
ZHAO Jiyong1, LI Lei2, WAN Yunyang1,2, LI Zhaoguo1, LUO Na2, MU Hongmei2, LI Wenhong1, ZHANG Yue3
(1.Exploration & Development Research Institute of PetroChina Changqing Oilfield Company, National Engineering Laboratory for Exploration and Development of Low-Permeability Oil & Gas Fields, Xi 'an 710018, China;2.College of Geosciences in China University of Petroleum(Beijing), State Key Laboratory of Petroleum Resource and Prospecting, Beijing Key Laboratory of Oil & Gas Pollution Control, Institute of Unconventional Oil and Gas Science and Technology, Research Centre for Geomicrobial Resources and Application, Beijing 102249, China;3.China National Offshore Oil Corporation Gas & Power Group, Beijing 100028, China)
Abstract:
Taking typical reservoir microorganism Bacillus sp. strain XJ02 as an example, the interaction between microbes and three minerals (dolomite, calcite, and mica) of oil reservoir was investigated. The interaction process was characterized by gas chromatography (GC), microbial analysis, pH value, redox potential(Eh), conductivity(σ), mineral quality, XRD and SEM-EDS, as well as the calculation software PHREEQC. The results show that XJ02 could increase the solubility of minerals, and the degree of solubilization is affected by mineral species (mica> calcite> quartz) and mineral particle size(0.074 mm> 0.900 mm). Minerals promotes the growth of XJ02 (mica> 0.074 mm calcite> 0.900 mm calcite> dolomite). The metabolic activities of XJ02 and its changes in environmental physical and chemical properties enhance the dissolution of minerals, which in turn effectively stimulates the growth of XJ02.
Key words:  Bacillus sp.  dolomite  calcite  mica  size effect  microorganisms  minerals